Heat transfer and thermoregulation within single cells revealed by transient plasmonic imaging

•A transient microscopic method for measuring thermal conductivity in single cells•Distinct living and dead cells normal cancer cells•First report of cell-level regulation according to ambient temperature•Cell metabolomic analysis unfold the mechanism cellular As basic unit life, carry out complex life activities all time. This activity is accompanied by continuous heat generation, transmission, dissipation. Heat control are therefore imperative maintain cells’ smooth operation. In this study, we demonstrated a visualize distribution conductivities cells. The different regions show wide heterogeneity. Distinct properties were observed between cells, warm- cold-blooded animal Importantly, first time, found cellular-level environmental temperature warm-blooded discovery will expand our knowledge about adaptability, thus may inspire research into internal active or passive as well biomedical applications using energy. 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(κ) room (0.6 W·m−1·K−1) reference value. basis proportion relationship (Equation 2). 0.29 W·m−1·K−1, literature (0.28 W·m−1·K−1).42Glycerine Producers' AssociationPhysical Properties Glycerine Solutions. Association, 1963Google evaluated effect experimental conditions results reliability air undetectable current 2A; Video S1) formed air, limit (approximately upto ±0.02) strong pump. addition, undetected when switched off without 2B). successfully tracked beams (Figures 1E S8; systems, power laser. linear 2C), theory (see details). dependence conditions, surface chemistry carefully investigated 2D 2E). showed final did depend conjugation, unlike usually highly ligands nanoparticles.29Baffou Particularly, induced slight κ, relatively (LPD) 0.038 mJ·cm−2 2D). condition attributed change correlations both theoretical calculation validation S4). minimize perturbation controlled subsequent measurements (LPD 0.016 mJ·cm−2), ΔT 4.3 K case, total 10–100 pJ irradiation, 0.0045 (10 pJ) ∼ 0.045 (100 whole Such tiny too induce any S1.3.3 details.) 2F (1/τ) water, glycerol, 50% glycerol/water, clear 1/τ. reported previous literatures.42Glycerine https://www.cell.com/cms/asset/ed8da210-eb2f-4025-8b05-65db7547135d/mmc2.mp4Loading ... Download .mp4 (14.03 MB) Help files S1. Dynamic (DF) immerged https://www.cell.com/cms/asset/9323f381-4720-491d-832a-d6299321469b/mmc3.mp4Loading (1.2 S2. raw 0.603